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Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing

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Journal of Apicultural Research Vol. 52 (5) pp. 235-241
DOI 10.3896/IBRA.1.52.5.10
Date December 2013
Article Title

Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing

Author(s)

Naser Tajabadi, Makhdzir Mardan, Mohd Yazid Abdul Manap and Shuhaimi Mustafa

Abstract

The objective of this study was to isolate and identify novel potential probiotic Lactobacillus using a culture method and Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene. SeventeenLactobacillus strains were isolated from a full honey comb of the honey bee Apis dorsata using selective media. The 16S rRNA genes from the extracted DNA of bacterial colonies were amplified with PCR using universal bacteria primers. All bacterial 16S rRNA genes were sequenced, aligned and the distant bacteria were deposited in GenBank. The lactobacilli strains were identified as Lactobacillus spp. related-sequences (64.15%) with other abundant sequences being related to Lactobacillus kunkeei (34.85%). The findings revealed that Apis dorsata honey comb has potential to be a source of new bacteria with probiotic activities in honey bee or as natural food preservatives for the food industry.

Keywords

 Lactobacillus, Apis dorsata, honey comb, identification, sequencing, 16S rRNA gene, probiotics

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