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Development of a simple and rapid method for typing population origins of the honey bee (Apis cerana) in Thailand

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Journal Name Journal of Apicultural Research
Article Title Development of a simple and rapid method for typing population origins of the honey bee (Apis cerana) in Thailand
Author(s) SC Imjongjirak, L Ngewsra, C Pramual, S Insuan, K Pala-or, S Klinbunga and S Sittipraneed
Volume 43
Issue 1
   
Published Date 2/1/2004
Pages 17 to 20
Abstract A simple and reliable method for determining the population origins of the honey bee (Apis cerana) in Thailand was developed based on restriction endonuclease analysis (REA) of genomic DNA with Hae III. Three profiles, A (9.6, 6.5 and 4.1 kb), B (12.0, 6.0 and 4.1 kb) and C (8.4, 5.7, 4.1 and 2.6 kb), were observed. Pattern A was found only in specimens originating from the northern Thailand population (north, north-east and the central region) whereas pattern B was observed in all bees from peninsular Thailand and 35% of those from Samui Island. Pattern C was not found in the mainland populations but restricted to bees from the Samui population (65%). Results indicated that REA- Hae III produced comparable results to PCR-RFLP of mtDNA gene segments, but were less tedious and more cost effective to perform.
Keywords honey bees, Apis cerana, DNA, restriction endonuclease analysis, REA, population typing, population origins, Thailand
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